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. 2016 Jun 9;6:27390. doi: 10.1038/srep27390

Figure 6. A2BR/CFTR FRET and cAMP production are dependent on an intact actin cytoskeleton.

Figure 6

BHK cells were exposed to vehicle (0.1% DMSO) or 10 μM cytochalasin D (CytD). Open bars are controls. Closed bars, adenosine (ADO). Grey bars, isoproterenol (ISO). (A) The ADO-induced increase in A2BRyfp–CFTRcfp FRET was abolished by cytochalasin D (n = 10). (B) β2ARyfp–CFTRcfp FRET is not agonist dependent and not affected by cytochalasin D (n = 12). (C) Cytochalasin D abolishes CFTR potentiation of ADO-induced cAMP production in BHKCFTR cells and is without effect in BHK cells (n = 12). (D) Cytochalasin D had no effect on ISO-induced increases in cAMP levels in BHKCFTR cells, but significantly reduced cAMP production in BHK cells. *p < 0.05 different from vehicle. †p < 0.05 different ± cytochalasin D (n = 12).