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. 2016 Jun 9;6:27390. doi: 10.1038/srep27390

Figure 7. The C-terminus of CFTR is required for CFTR-A2BR interactions at the plasma membrane.

Figure 7

(A) Typical pre-and post-bleach images obtained by confocal microscopy of gfp-tagged CFTR (green) and mCherry-tagged A2BR (red) expressed in BHK cells. Scale bar is 25 μm. (B) Mean FRET efficiency data. Left, plasma membrane FRET measured between A2BRmCherry and either wild-type CFTRgfp or the L1480A CFTRgfp mutant under basal or adenosine-stimulated conditions. All n = 7–9. Right, intracellular FRET between CFTRgfp and A2BRmCherry ± adenosine. Open bars, vehicle. Closed bars, FRET after 5 min exposure to 10 μM adenosine. (C) Intracellular cAMP levels under basal conditions (open bars) and following 5 min adenosine exposure (closed bars) in BHK cells endogenously expressing A2BR and either no CFTR, CFTR tagged with gfp on its C-terminus, extracellularly HA-tagged CFTR and following mutation of the C-terminus (L1480A). All n = 6. NT is non-transfected control. *p < 0.05 different from vehicle. †p < 0.05 different to wild-type or different ± CFTR.