Figure 2. Codon optimization of the fiber increases its expression in transfected cells.

(A) CAI comparison between the wild-type adenoviral fiber (WT Fiber, red) and the newly engineered codon optimized fiber (OPT Fiber, black). CAI values of each codon are represented raw and smoothened for both proteins. (B) Representative western blot of WT and OPT tagged fibers in A549, HeLa and RPE-1 cells at 48 h post transfection. (C) Absolute quantification of plasmid DNA content per cell by qPCR. (D) qPCR analysis of WT and OPT fiber mRNA levels in the indicated cells. Data is shown as mean ± SEM of four independent experiments. *p < 0.05, ***p < 0.001. Fibers mRNA values are expressed as relative to cellular β-actin in each replicate. *p < 0.05, **p < 0.01.