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. 2016 Jun 9;6:27546. doi: 10.1038/srep27546

Figure 3. Fiber codon optimization in the adenovirus reduces expression of viral structural proteins and attenuates viral production.

Figure 3

(A) Schematic representation of wild-type (Adwt) and fiber-optimized (AdFO) adenoviral genomes. (B) Graphic description of the time-course of key adenoviral events in infected cells. (C) Representative western blot of hexon and fiber protein expression at indicated times. (D) Viral mRNA content analyzed at early (12 h), mid (24), and late (30/36 h) phases post infection. Relative AdFO hexon (green) and fiber (pink) mRNA content is shown as mean ± SEM of four independent experiments. Fiber mRNA increase over time is indicated with a grey dotted line. (E) Quantification of intracellular viral DNA content by qPCR. (F) Extracellular viral DNA release analyzed by qPCR at 30 h and 72 h. Cells were infected using 10 TU/cell of both viruses. Data is shown as mean ± SEM of five independent experiments. All AdFO DNA, mRNA and viral release values are expressed as relative to their corresponding value for Adwt in each replicate. *p < 0.05, **p < 0.01.