Table 3. RT-PCR Forward/Reverse (F/R) Primers Sequences.

Forward/Reverse	Sequence (5′-3′)
BNP	F: 5′-GACAAGAGAGAGCAGGACACCAT-3′
	R: 5′-TAAGGAAAAGCAGGAGCAGAATCAT-3′
TGF-β	F: 5′-CTAATGGTGGACCGCAACAAC-3′
	R: 5′-CACTGCTTCCCGAATGTCTGA -3′
TNF-а	F: 5′-AGCAAACCACCAAGCGGAGG-3′
	R: 5′-CAGCCTTGTCCCTTGAAGAGAAC-3′
MCP-1	F: 5′-TCTGTGCTGACCCCAATAAGGAA-3′
	R:5′-GAGGTGGTTGTGGAAAAGAGAGTG-3′
Cys-C	F: 5′-CCACCAGGAGACAGTAAAGAAGC-3′
	R: 5′-ATTGAGCAAGAGCAAGGTATGAC-3′
NGAL	F: 5′-GGCTGTCGCTACTGGATCAGA-3′
	R: 5′-GCTTGGTGGAATCATGGCTGG-3′
а-SMA	F: 5′-CTCCCAGCACCATGAAGATCAA-3′
	R: 5′-GGGCGTGACTTAGAAGCATTTG-3′
CRP	F: 5′-AAGCCTTCACTGTGTGTCTCTATGC-3′
	R: 5′-TTCAGGCCCACCTACTGCAATA-3′
IL-6	F: 5′-AGTTGCCTTCTTGGGACTGATGT-3′
	R: 5′-GGTCTGTTGTGGGTGGTATCCTC-3′
IL-1β	F: 5′-ATGTATGCCTACTCATCGGGA-3′
	R: 5′-CAACACAGGCTTGTCTTCTCC-3′
GAPDH	F: 5′-CAACGGGAAACCCATCACCA-3′
	R: 5′-ACGCCAGTAGACTCCACGACAT-3′

BNP, brain natriuretic peptide; TGF-β, transforming growth factor-β; TNF-α, tumor necrosis factor-α; MCP-1, monocyte chemotactic protein 1; Cys-c, Cystatin C; NGAL, neutrophil gelatinaseassociated lipocalin; α-SMA, α-smooth muscle actin; CRP, C-reaction protein; IL-6, interleukin-6; IL-1β, interleukin-1β; GAPDH, glyceraldehydes-3-phosphate dehydrogenase.