Skip to main content
. 2016 Jun 9;6:27729. doi: 10.1038/srep27729

Figure 2. In vivo functional assay of IL-1β mutants.

Figure 2

(a) Cortisol production is induced in a dose-dependent manner by chicken IL-1β and released into the plasma. (b) To determine the bioactivities of WT chicken IL-1β (ChIL-1β), T7A, R8A, N18A, E25A, H34A, Q36A, R52A, R54A, Q64A, T117A, E118A, E118K, E118R, and Q138A, and human IL-1β (HmIL-1β), each protein was directly and individually injected into a wing vein of three adult chickens, after which the plasma cortisol levels were measured. (c) Ribbon diagram of chicken IL-1β (grey) complexed to IL-1R (pink). Mutation sites are shown in blue. Mutated residues in site A, site B, and loop 9 are shown in (d–f), respectively with the cortisol levels relative to that of WT IL-1β shown to the right of the mutant names.