Skip to main content
. 2016 Jun 9;6:27729. doi: 10.1038/srep27729

Figure 4. Surface plasmon resonance assays to determine the binding affinities of WT chicken IL-1β and its mutants to chicken IL-1RI.

Figure 4

(a) WT chicken IL-1β, (b) T117A, and (c) E118K. Sensorgrams were obtained by injecting various concentrations of WT IL-1β or a mutant over a chip with surface-immobilized IL-1RI. The experimental data (red lines) were fit with a 1:1 interaction model (black lines). RU, response unit.