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. 2016 Jun 9;6:27667. doi: 10.1038/srep27667

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Copyright © 2016, Macmillan Publishers Limited

This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/

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Strain JHY617-SDN was cultivated in YPD medium containing 100 g/L glucose with initial OD₆₀₀of 9.5. Glucose was intermittently added into culture medium using the feeding solution (800 g/L glucose) before glucose was completely consumed.