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. 2016 Jun 9;6:27667. doi: 10.1038/srep27667

Table 1. Fermentation characteristics of recombinant strains.

Strain Description Fermentation time (h) Cell density (OD600) Consumed glucose (g/L) Products (g/L)
Productivity of acetoin (g/(L·h)) Yield of acetoin (g/g glucose)
Ethanol Glycerol 2,3-BDO Acetoin
Batch flask fermentation in SC-His medium
 JHY605-C adh1-5Δgpd1Δgpd2Δ [EV] 96 6.89 ± 1.07 31.6 ± 4.64 3.8 ± 0.12 0.01 ± 0.00 1.37 ± 0.10 0.13 ± 0.02 0.001 ± 0.000 0.004 ± 0.000
 JHY605-SD adh1-5Δgpd1Δgpd2Δ [SD] 72 11.29 ± 0.15 51.5 ± 0.16 1.14 ± 0.12 0.02 ± 0.00 9.30 ± 0.14 5.87 ± 0.84 0.082 ± 0.012 0.114 ± 0.016
 JHY617-SD adh1-5Δgpd1Δgpd2Δbdh1Δ [SD] 72 12.42 ± 0.50 51.1 ± 0.04 0.89 ± 0.06 0.01 ± 0.00 0.18 ± 0.16 15.43 ± 0.49 0.214 ± 0.007 0.302 ± 0.007
 JHY617-SDN adh1-5Δgpd1Δgpd2Δbdh1Δ [SDN] 48 10.76 ± 0.20 51.5 ± 0.04 0.58 ± 0.13 0.01 ± 0.00 0.14 ± 0.01 20.13 ± 1.02 0.419 ± 0.021 0.391 ± 0.020
Fed-batch fermentation in YPD medium
 JHY617-SDN adh1-5Δgpd1Δgpd2Δbdh1Δ [SDN] 55 28.25 227.7 0.40 0.34 0.39 100.08 1.820 0.439