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. 2016 Jun 9;6:27578. doi: 10.1038/srep27578

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Copyright © 2016, Macmillan Publishers Limited

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EsCrt mRNA and protein expression profile in the hepatopancreas after LPS (A), PGN (B), S. aureus (C), and V. parahaemolyticus (D) challenge as measured by qRT-PCR and western blot. GAPDH gene was used as an internal control to calibrate the cDNA template for all of the samples. Vertical bars represent mean ± S.E. (N = 5). Each bar represents the mean value of five determinations with standard deviation. Asterisks indicate significant differences (*P < 0.05, **P < 0.01, ***P < 0.001) compared with the values of control. The β-actin was used as internal control to calibrate the protein template of all samples.