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. 2016 Jun 9;6:27789. doi: 10.1038/srep27789

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Copyright © 2016, Macmillan Publishers Limited

This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/

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Raman images constructed from MCR analysis: (A) S. pombe vegetative cells and (B) ascospores. MCR component distributions images from of (1) Lipid [gold arrows indicate lipid accumulation], (2) protein, (3) glucans, (4) mannan, (5) polyphosphates (PolyP) and correlation profiles between glucan and mannan showing colocalization in spores. Corresponding bright field optical images are included for reference.