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. 2004 Jul 12;101(29):10602–10607. doi: 10.1073/pnas.0404100101

Fig. 2.

Fig. 2.

Stat-3C-dependent induction of MMP-9 mRNA, luciferase activity, and protein. (A) Induction of MMP-9 mRNA in pBabe-Stat3-C (pB-3C)- and pBabe (pB)-infected MCF-10A and HMLHT cells determined by RT-PCR (Upper), normalized to GAPDH (Lower). (B) HMLHT cells were transfected with an MMP-9 promoter luciferase construct in conjunction with either pBabe (pB)- or pB Stat3-C (3-C)-expressing plasmids. Luciferase activities are shown as the mean ± SD of three experiments performed in duplicate. (C) MMP-9 protein expression in cell culture medium from pBabe (pB)- and pBabe-Stat3-C (pB-3C)-infected MCF-10A and HMLHT cells shown by gelatin zymography.