Skip to main content
. Author manuscript; available in PMC: 2016 Jun 9.
Published in final edited form as: Microb Pathog. 2014 Apr 24;73:70–79. doi: 10.1016/j.micpath.2014.04.005

Fig. 1.

Fig. 1

FSTL-1 hypomorphic mice have decreased fstl-1 gene transcription and FSTL-1 serum protein. (A). A 2565 bp insert containing loxP sequence was inserted within the fstl-1 exon 1 along with flP, a neomycin cassette, a second flP and second loxP site, and was (B) detectable by PCR as FSTL-1 hypomorphic mice yielded a 238 bp gene product (hypo) while wild-type (WT) mice yielded a 199 bp band; in heterozygotes (het) both gene products were detectable. (C) This insertion resulted in FSTL-1 hypomorphic mice with a 60% reduction in serum FSTL-1 (D). Hypomorphic mice showed a reduction in joint fstl-1 transcription versus wild-type mice at day 14 and 42 post infection. Data are representative of at least two individual experiments with n = 10 mice/treatment group. **p < 0.01, ***p < 0.001, ****p < 0.0001.