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. 2016 Jun 9;13:30. doi: 10.1186/s12989-016-0142-8

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© The Author(s). 2016

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 4 — Measurement of total IgE and C5a in serum or BALF after intratracheal instillation of NiO NPs at 200 cm²/rat. Total IgE levels were measured in (a) serum and (b) BALF. Levels of C5a were measured in (c) serum and (d) BALF. Abbreviations: VEH_NP, vehicle control for NiO NPs; OVA, ovalbumin-induced allergic airway inflammation; VEH_OVA, vehicle control for OVA group. Mean ± SEM (n = 4). Student’s t-test was applied for comparison between OVA group and vehicle control (VEH_OVA) with statistical significance indicated by ^### p < 0.001