Table 1. Oligonucleotides primers used for RT-qPCR in this study.
| Target | 5’ – 3’ | Sequence | Gene ID |
|---|---|---|---|
| SREBP-1c | Forward | GATGTGCGAACTGGACACAG | 6720 |
| Reverse | CATAGGGGGCGTCAAACAG | ||
| SCD1 | Forward | CCCCTGCGGATCTTCCTTAT | 20249 |
| Reverse | AGGGTCGGCGTGTGTTTCT | ||
| ACC1 | Forward | CCATTGGTATTGGGGCTTAC | 107476 |
| Reverse | CCCGACCAAGGACTTTGTTG | ||
| FAS | Forward | GCTGCGGAAACTTCAGGAAAT | 14102 |
| Reverse | AGAGACGTGTCACTCCTGGACTT | ||
| PPARγ | Forward | AGTGGAGACCGCCCAGG | 19016 |
| Reverse | GCAGCAGGTTGTCTTGGATGT | ||
| DGAT2 | Forward | AGTGGCAATGCTATCATCATCGT | 67800 |
| Reverse | AAGGAATAAGTGGGAACCAGATCA | ||
| PPARα | Forward | ATGCCAGTACTGCCGTTTTC | 19013 |
| Reverse | GGCCTTGACCTTGTTCATGT | ||
| ACO | Forward | GCCCAACTGTGACTTCCATT | 74121 |
| Reverse | GGCATGTAACCCGTAGCACT | ||
| CPT1 | Forward | GCACTGCAGCTCGCACATTACAA | 12894 |
| Reverse | CTCAGACAGTACCTCCTTCAGGAAA | ||
| Nrf2 | Forward | CGAGATATACGCAGGAGAGGTAAGA | 18024 |
| Reverse | GCTCGACAATGTTCTCCAGCTT | ||
| β-actin | Forward | CTGTCGAGTCGCGTCCA | 11461 |
| Reverse | CTCGCGGGTGGACGCGACTCGACAG |
SREBP-1c, Sterol regulatory element-binding protein-1c; SCD1, Stearoyl-CoA desaturase 1; ACC1, Acetyl-CoA carboxylase 1; FAS, Fatty acid synthase; PPAR, Peroxisome proliferator-activated receptor; DGAT2, Diacylglycerol acyltransferase 2; ACO, Acyl-CoA oxidase; CPT1, Carnitine palmitoyltransferase 1; Nrf2, NF-E2-related factor-2