Fig. 1.

Random spore analysis. mad1Δ, mad2Δ, mad3Δ, and ura3Δ strains exhibit colony growth indistinguishable from the deletion collection parental strain (data not shown). ura3Δ ykoΔ double mutants were evaluated to reveal any slow-growth phenotypes of ykoΔ mutants (notably bim1Δ and kar3Δ). (A) Growth of mutants in the outer kinetochore Ctf19 complex in combination with mad1Δ and ykoΔ interacting pairs identified is shown. Also shown are the growth phenotypes of these ykoΔ mutants in combination with mad2Δ and mad3Δ. madΔ ykoΔ double mutants that do not grow have a synthetic lethal interaction (SL). Synthetic fitness (SF) interactions are seen as reduced colony size in the madΔ ykoΔ double mutant versus ura3Δ ykoΔ. Double mutants with no distinguishable growth defect are considered fine (F). (B) The growth phenotypes of the mutants that affect microtubule stability are shown in combination with ura3Δ, mad1Δ, mad2Δ, and mad3Δ. Random spore analysis could not be used to evaluate cin8Δ in double mutant combinations because cin8Δ and can1Δ are closely linked. The previously reported synthetic lethal interactions with cin8Δ (15) were therefore confirmed only by tetrad dissection (Table 3). (C) Examples of tetrad dissections of heterozygous madΔ ykoΔ diploids. Double mutants (inferred or observed) are indicated.