Table 1.
Primer name | Sequence (5′–>3′) | Amplicon size (bp) | PCR efficiency (%) |
---|---|---|---|
S4TE EFFECTORS | |||
CKC_RS05675 F | TGTCTCATTCCGTTGCTTCC | 104 | 93.2 |
CKC_RS05675 R | CCAATGCCACACTCCGTAATA | ||
CKC_RS04080 F* | GTTACGCCTTGTAGATCCAGAG | 104 | 95.2 |
CKC_RS04080 R* | CTCGCTCTATTTCCTCCGTTATT | ||
CKC_RS03550 F* | CTTTTGCACGCATTAGCAG | 144 | 98.7 |
CKC_RS03550 R* | AACTTCTTCCGGAACACTC | ||
CKC_RS05565 F | AATGCTGTTTCTGGGGTTG | 130 | 90.2 |
CKC_RS05565 R | CTAGAGTTAGAACATGCGG | ||
CKC_RS01780 F | TGGACGTGGTGTTTCCTATTT | 131 | 88.3 |
CKC_RS01780 R | CCTGCATTTCATGCGCTAATC | ||
CKC_RS04955 F | AACGGTTCCTCAGGTGGTT | 133 | 93.4 |
CKC_RS04955 R | GCTTCAGCTGTTGTAGCTT | ||
CKC_RS03370 F | ATGGCTTCTAGGCGTGTTT | 101 | 93.1 |
CKC_RS03370 R | CGCCCTCCTCTAACTTGTAATC | ||
CKC_RS01290 F | AGAACCTGCTCCAGGAATAAAG | 92 | 91.3 |
CKC_RS01290 R | CTGCAACACGTGCTGAAATAG | ||
CKC_RS05235 F | GATCGTCCAAACACATGGATAAAC | 91 | 87.2 |
CKC_RS05235 R | TCCATGCTTCTATGCTGTGAG | ||
CKC_RS03655 F | CTCGTCGTCTTGCTGCTATT | 102 | 92.7 |
CKC_RS03655 R | GCAGATGTGCTTTCATAAGTTCC | ||
CKC_RS02175 F | TCGGCTATATCCAGCCAAATATC | 109 | 89 |
CKC_RS02175 R | GATCACCATTGATCTTCCGTAGT | ||
CKC_RS01950 F | GTCGCGCGGGAAGTAATAAA | 96 | 93.6 |
CKC_RS01950 R | CATGTTCGGCTTCTCGGAAATA | ||
CKC_RS03915 F* | CAATTCCATCAACGCAAG | 90 | 92.8 |
CKC_RS03915 R* | TTTCCGCTGGAGTAGCTT | ||
CKC_RS00880 F | CACAGCATCTTCCTGAGTTAGT | 96 | 93.7 |
CKC_RS00880 R | TCTAATCCTCCACGGTGAAATG | ||
CKC_RS00225 F | TGATGCGCGTGGTATTAGAG | 103 | 94.5 |
CKC_RS00225 R | GGCCAAGAAGGGAAGGAATA | ||
CKC_RS00885 F | GGAGGTTGAAGCTCTTAAG | 141 | 91.7 |
CKC_RS00885 R | TTGGAATCAAACTCTGCCC | ||
EFFECTOR WEBSITE | |||
CKC_RS04230 F* | GTCTAAGTTGCGAATTGCC | 149 | 100 |
CKC_RS04230 R* | AAAAGTCTCGGGTTCATCC | ||
CKC_RS02605 F | TCATGGGTCGTGCTATGAT | 159 | 92.7 |
CKC_RS02605 R | AGCCAAAGACATGCTCTTC |
primers used to validate bioinformatic analysis of transcriptomic data.