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. 2004 Jul 12;101(29):10703–10708. doi: 10.1073/pnas.0403652101

Fig. 5.

Fig. 5.

Reduced lipid accumulation in PPARγ2-/- MEFs. (A) At day 9 after induction of adipocyte differentiation, cells were fixed and stained for neutral lipids with Oil red O. (B) Either PPARγ1 or PPARγ2 promotes the in vitro adipogenesis of PPARγ2-/- MEFs. We generated the PPARγ2-KO MEF-based cell lines by stable transfection with the puromycin resistance vector (pQCXIP), pQCXIP containing mouse wild-type PPARγ1, or PPARγ2 cDNA. At day 9 after induction, cells were fixed and stained for neutral lipids with Oil red O (×200).