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. 2004 Jul 12;101(29):10709–10714. doi: 10.1073/pnas.0402492101

Fig. 2.

Fig. 2.

Effects of actinomycin d on the localization of nucleolar proteins. HeLa cells were treated with 50 ng/ml actinomycin d for 2 h and stained by indirect immunofluorescence. Nuclei with dark-phase nucleoli are shown. (A) Treacle (red) localization in untreated and treated cells is compared with RNA helicase II/Guα (green). (B) The localizations of full-length treacle and its C terminus both fused to GFP (green) are compared with endogenous UBF (red) in actinomycin d-treated cells. (C) Enlarged view of a superimposed phase and GFP fluorescence of actinomycin d-treated HeLa cell transfected with full-length treacle–GFP construct.