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. 2004 Jul 12;101(29):10709–10714. doi: 10.1073/pnas.0402492101

Fig. 5.

Fig. 5.

Analysis of RNA from mouse embryos. RT-PCR was used to determine the levels of expression of Tcof1 in embryonic day 10.5 heterozygous (+/-) embryo relative to its WT (+/+) littermate. (Left) Tcof1 signals were normalized by using U1C as an internal control. (Right) The level of pre-rRNA was determined by RNase protection assay using a 5′ pre-rRNA probe and β-actin as an internal control. Similar results were obtained with another set of embryos.