Figure 1.

HPLC overlay chromatogram of Bruguiera gymnorrhiza (BRG), n-butanol fraction of BRG and compound G. The HPLC fingerprint indicated the presence of compound G at retention time of 15.12 min. The analysis was carried out using Shimadzu high-performance liquid chromatography system attached with an ultraviolet detector and prepacked C18 column (4.6 mm × 250 mm, 5 μm; Agilent Technologies). The separation was carried out using a gradient conc. of Solvent A (Water with 0.1% TFA) and Solvent B (Acetonitrile with 0.1% TFA). The total run time for the separation was 30 min (flow rate of 1 ml/min) at λ = 220 nm