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. 2004 Jul 9;101(29):10732–10737. doi: 10.1073/pnas.0404117101

Fig. 3.

Fig. 3.

Developmental regulation of vasorin. (A) Northern blot analysis of staged mouse embryos (E10.5, E13.5, and E17.5). (B) Expression pattern of vasorin during aortic development examined by in situ hybridization analyses. The fourth image is the corresponding bright-field image of the third representation. Arrowheads indicate the aorta in the mouse embryo (E17.5). (C) Semiquantitative RT-PCR comparing the expression of vasorin in the adult rat aorta with that in cultured rat aortic VSMCs. Rat α-smooth muscle actin (α-SMA) and 18S rRNA were used as a positive and an internal control, respectively. (D) Semiquantitative RT-PCR showing the induction of the vasorin gene in RA-treated A404 cells. Rat α-SMA and 18S rRNA were used as a positive and an internal control, respectively.