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. 2004 Jul 9;101(29):10750–10755. doi: 10.1073/pnas.0404044101

Fig. 1.

Fig. 1.

Generation of Ncb5or -/- mice. (a) Schematic representation of the Ncb5or wild-type allele, knockout targeting construct and targeted allele. The BsgI and BglII sites and P1 and P2 probes were used to genotype the mice by Southern blots. The arrows represent the primers used in genotyping by PCR. (b) Genotyping of mice by multiplex PCR. The primers for wild-type allele (WT) amplify a 366-bp product, and the primers for knockout allele (KO) amplify a 427-bp product. (c) Western blot analysis of NCB5OR protein (arrow) in pancreata. The upper band represents a nonspecific protein. (d) RT-PCR analysis of expression of Ncb5or mRNA in isolated islets of Ncb5or +/+ mice. (e) Northern blot (Left) and RT-PCR (Right) analyses of Ncb5or mRNA in livers and kidneys of Ncb5or +/+ (WT), -/- (KO), and +/- (HT) mice. The mRNA detected in the +/+ mice was derived from the wild-type allele. The mRNA detected in -/- mice was derived from the knockout allele, which lacks the entire exon 4.