Table 1. PCR and extension primer sequences.

Mutation	CD 41/42 (-CTTT)	IVS2 654 (C → T)	nt —28 (A → G)	CD 17 (A → T)	rs2187610
PCR primer 1	5′-ACGTTGGATGTAACAGCATCAGGAGTGGAC-3′	5′-ACGTTGGATGTAACAGTGATAATTTCTGGG-3′	5′-ACGTTGGATGTAGGGTTGGCCAATCTACTC-3′	5′-ACGTTGGATGTCACCACCAACTTCATCCAC-3′	5′-ACGTTGGATGATGCCATTTCATGGTTACC-3′
PCR primer 2	5′-ACGTTGGATGCTATTTTCCCACCCTTAGGC-3′	5′-ACGTTGGATGGAAACCTCTTACATCAGTTAC-3′	5′-ACGTTGGATGAGCAATAGATGGCTCTGCCC-3′	5′-ACGTTGGATGTCAAACAGACACCATGGTGC-3′	5′-ACGTTGGATGGAAGTGAGGCTACATCAAAC-3′
Standard protocol
Extension primer	5′-GATCCCCAAAGGACTCAA-3′	5′-TGATAATTTCTGGGTTAAGG-3′	5′-AGCCAGGGCTGGGCATA-3′	5′-TTCATCCACGTTCACCT-3′	5′-ACCTTTCATTTGTTCATTGTTTT-3′
Terminator mix*	CGT	AC	AC	CGT	ACT
Expected molecular weight of extended nonmutant allele	6,088	6,475	5,558	5,345	7,225 (G allele)
Expected molecular weight of extended mutant allele	5,735	6,804	5,887	5,683	7,569 (C allele)
SABER protocol
Extension primer	5′-GATCCCCAAAGGACTCAA-3′	5′-ATATGCAGAAATATTGCTATT-3′	5′-GATGGCTCTGCCCTGACTT-3′	5′-TTACTGCCCTGTGGGGC-3′	5′-ACCTTTCATTTGTTCATTGTTTT-3′
Terminator	ddCTP	ddATP	ddCTP	ddTTP	ddCTP
Expected molecular weight of nonextended primer	5,462	6,443	5,771	5,193	6,952
Expected molecular weight of extended allele	5,735	6,741	6,044	5,482	7,225 (G allele)

^*CGT mix is ddCTP/ddGTP/ddTTP/dATP in which dd indicates the 2′,3′-dideoxynucleoside. Similarly, AC mix is ddATP/ddCTP/dGTP/dTTP