Skip to main content
. 2004 Jul 12;101(29):10774–10779. doi: 10.1073/pnas.0402361101

Fig. 2.

Fig. 2.

Human TRIM5α is necessary and sufficient to confer Ref1 activity. (A) Infection of MDTF cells that were unmodified (None) or expressing either of two human TRIM5α variants (hu.1 and hu.2) by N-MLV-GFP (filled bars) or B-MLV-GFP (open bars). The effects of Fv1n and Fv1b expression on N-MLV and B-MLV infection is shown for comparison. (B) Silencing of TRIM5αhu expression by using siRNA. HeLa cells were cotransfected with plasmids expressing HA-TRIM5α and GFP in the absence of siRNA (-) or in the presence of siRNAs targeting luciferase (Luc) or TRIM5αhu (lanes A and B). (C) Infection of human (HeLa) cells with N-MLV or B-MLV after transfection with either no siRNA, the control-luciferase-specific siRNA, or the TRIM5-specific siRNA-A, as indicated. (D) Effects of control (Luc) and TRIM5-specific siRNAs (A and B) on N-MLV (filled bars) and B-MLV (open bars) infection of HeLa cells.