Fig. 3.

Restriction of MLV by Trim5α. The graphs on the left show titration curves for a panel of MLVs on TE671 (A) and Vero (C) cell lines. Cells were infected with increasing amounts of viruses (previously titrated on M. dunni cells). Viruses tested were N-MLV (♦, solid line), B-MLV (▴, solid line), NB-MLV (▪, solid line), N-N82D (♦, dashed line), N-L117H (▪, dashed line), and B-E110A (▴, dashed line). The titrations come from one typical experiment. The plots in the right column show the fold reduction (percentage of Trim5α+ cells infected/percentage of Trim5α- cells infected) in MLV titer as measured by the two-color FACS assay upon introduction of human (B) and AGM (D) Trim5α into M. dunni cells. Means and SD from three experiments are shown.