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Effects of Trim5α siRNA on restriction in primate cells. (A) Titration curves of MLV and HIV-1. ▪, TE671: N-MLV; ▪, B-MLV. Continuous lines show titers in the absence of siRNA and broken lines show titers in the presence of siRNA. (B) PCR of 5-fold serial dilutions of Trim5α cDNA isolated from the cells in A. GAPDH was used as a negative control. Data from one representative experiment are shown.
