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. 2004 Jul 12;101(29):10798–10803. doi: 10.1073/pnas.0402397101

Fig. 1.

Fig. 1.

Influence of pilV on expression of the pilin PC epitope. Lanes: 1, N400 (wild type); 2, GV1 (pilVfs); 3, GV5 (pilVfs, iga::pilV); and 4, GV37 (pilVoe). (A) Coomassie-stained SDS/PAGE gel loaded with whole-cell lysates showing the relative migration of PilE. (B) Immunoblotting of whole-cell lysates by using monoclonal antibody TEPC-15 (1:1,000). (C) Coomassie-stained SDS/PAGE gel showing the relative amounts of PilE in purified pili. (D) Immunoblotting of purified pili by using monoclonal antibody TEPC-15. With regard to PilV expression, + denotes the wild-type allele, - denotes the PilVfs-null allele, -/+ denotes complementation of a pilV-null mutation by means of ectopic expression, and ++ denotes the pilVoe allele.