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MS/MS of wild-type, modified PilE tryptic peptides. (A) Characterization of the species at [M + 2H]2+ (strains N400 and MW25). The peptide 57WPENNTSAGVASPPTDIK74 is modified with PE and the disaccharide HexDATDH. (B) Characterization of the modified PilE thermolysin/tryptic peptide at m/z 525 [M + 2H]2+ (strain N400). The peptide 66VASPPTDIK74 is modified with PE. Fragmentation patterns are shown in A Upper and B Upper.
