Table 1. K_m and V_max Values for Wild-Type CaN at 37 °C with pNPP as a Substrate.

	Km (mM)	Vmax (μmol min–1 mg–1)
dilute buffer	56 ± 8	1.4 ± 0.1
dextran 70
10 g/L	36 ± 8	1.2 ± 0.11
25 g/L	26 ± 4	1.0 ± 0.10
50 g/L	22 ± 2	1.6 ± 0.05
100 g/L	21 ± 2	1.7 ± 0.05
150 g/L	23 ± 5	1.2 ± 0.09
200 g/L	17 ± 3	1.1 ± 0.07
dextrose
50 g/L	59 ± 12	1.1 ± 0.11
100 g/L	56 ± 5	0.95 ± 0.04
150 g/L	70 ± 8	0.98 ± 0.06
200 g/L	58 ± 18	1.6 ± 0.2
ficoll 70
50 g/L	38 ± 5	0.92 ± 0.10
100 g/L	10 ± 2	0.83 ± 0.10
150 g/L	19 ± 7	0.99 ± 0.12
200 g/L	9.2 ± 6	1.4 ± 0.3
sucrose
50 g/L	69 ± 8	2.1 ± 0.1
100 g/L	59 ± 4	1.5 ± 0.1
150 g/L	76 ± 11	1.5 ± 0.1
200 g/L	63 ± 5	1.3 ± 0.1