Table 1. Km and Vmax Values for Wild-Type CaN at 37 °C with pNPP as a Substrate.
Km (mM) | Vmax (μmol min–1 mg–1) | |
---|---|---|
dilute buffer | 56 ± 8 | 1.4 ± 0.1 |
dextran 70 | ||
10 g/L | 36 ± 8 | 1.2 ± 0.11 |
25 g/L | 26 ± 4 | 1.0 ± 0.10 |
50 g/L | 22 ± 2 | 1.6 ± 0.05 |
100 g/L | 21 ± 2 | 1.7 ± 0.05 |
150 g/L | 23 ± 5 | 1.2 ± 0.09 |
200 g/L | 17 ± 3 | 1.1 ± 0.07 |
dextrose | ||
50 g/L | 59 ± 12 | 1.1 ± 0.11 |
100 g/L | 56 ± 5 | 0.95 ± 0.04 |
150 g/L | 70 ± 8 | 0.98 ± 0.06 |
200 g/L | 58 ± 18 | 1.6 ± 0.2 |
ficoll 70 | ||
50 g/L | 38 ± 5 | 0.92 ± 0.10 |
100 g/L | 10 ± 2 | 0.83 ± 0.10 |
150 g/L | 19 ± 7 | 0.99 ± 0.12 |
200 g/L | 9.2 ± 6 | 1.4 ± 0.3 |
sucrose | ||
50 g/L | 69 ± 8 | 2.1 ± 0.1 |
100 g/L | 59 ± 4 | 1.5 ± 0.1 |
150 g/L | 76 ± 11 | 1.5 ± 0.1 |
200 g/L | 63 ± 5 | 1.3 ± 0.1 |