|
| ||
| Component | Amount (μl) | Final concentration |
|
| ||
| Tagmented DNA (from step 20 B(ix)) | 25 | |
|
| ||
| Nextera PCR Master Mix | 15 | |
|
| ||
| Index 2 Primer | 5 | |
|
| ||
| Index 1 Primer | 5 | |
|
| ||
| Final | 50 (for one reaction) | |
|
| ||
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