|
| ||
| Component | Amount (μl) | Final concentration |
|
| ||
| Diluted cDNA | 68 | |
|
| ||
| NEBNext Second Strand Synthesis Reaction Buffer (10×) | 8 | 1× |
|
| ||
| NEBNext Second Strand Synthesis Enzyme Mix | 4 | |
|
| ||
| Final | 80 (for one reaction) | |
|
| ||
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