|
| ||
| Component | Amount (μl) | Final concentration |
|
| ||
| Q5 Reaction Buffer (5×) | 10 | 1× |
|
| ||
| dNTPs (10 mM each) | 1 | 0.2 mM each |
|
| ||
| Forward primer (25 μM) | 1 | 0.5 μM |
|
| ||
| Reverse primer (25 μM) | 1 | 0.5 μM |
|
| ||
| cDNA (from Step 19) | 5 | |
|
| ||
| Q5 Hot-Start DNA Polymerase (2 U/μl) | 0.5 | 0.02 U/μl |
|
| ||
| Nuclease-free water | 31.5 | |
|
| ||
| Final | 50 (for one reaction) | |
|
| ||
Official websites use .gov
A
.gov website belongs to an official
government organization in the United States.
Secure .gov websites use HTTPS
A lock (
) or https:// means you've safely
connected to the .gov website. Share sensitive
information only on official, secure websites.