Fig. 3. SRS microscopy of tissue at the periphery of high- and low-grade gliomas.

(A) SRS images of the margin of an infiltrating glioblastoma within cortex depicting a transition from densely tumor-infiltrated brain to minimally infiltrated brain (left to right). (B to D) Cellularity and protein:lipid ratio vary in high-magnification images acquired in densely infiltrated tissue (B), moderately infiltrated tissue (C), and minimally infiltrated tissue (D). (E) SRS imaging of an oligodendroglioma infiltrating within white matter, depicting a transition from densely tumor-infiltrated brain to minimally infiltrated brain (left to right). (F to H) Variation in axonal density, cellularity, and protein:lipid ratio is apparent when comparing high-magnification images from densely infiltrated tissue (F), moderately infiltrated tissue (G), and minimally infiltrated tissue (H).