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. Author manuscript; available in PMC: 2016 Nov 1.
Published in final edited form as: Ann Neurol. 2015 Sep 25;78(5):787–800. doi: 10.1002/ana.24517

Figure 3.

Figure 3

[F-18]-AV-1451 nuclear emulsion autoradiography photomicrographs of brain slices from an AD case (A–C), a CAA carrier of the D23N Iowa APP mutation (D), a rTg4510 mouse (E), a PiD case (F), a PSP case (G) a CBD case (H), a DLB case (I), a MSA case (J) and a FTDL-TDP-43 case (K). Combined [F-18]-AV-1451 nuclear emulsion autoradiography followed by immunostaining with appropriate antibodies (B right and left panels, C–K) or H&E counter staining (B middle panel). The primary antibodies used were PHF-1 (kind gift of Dr. Peter Davies, A middle panel, B left and right panels, and E–H), anti-Aβ (A right panel, C and D), anti α-synuclein (I and J) and anti-TDP-43 (K). [F-18]-AV-1451 nuclear emulsion autoradiography showed a strong cortical accumulation of silver grains in cortical layers III and V in AD brains (A, left panel) mirroring the pattern on adjacent slices of tau PHF-1 immunostaining (A, middle panel) but not the Aβ immunostaining (A, right panel). Accumulation of silver grains from the nuclear emulsion colocalized with PHF-1 stained tangles (solid arrows, B, left panel), both intraneuronal (solid arrows) and extraneuronal (black arrowheads, B middle panel) and PHF-tau containing neurites surrounding plaques in AD brains (empty arrows, B, right panel). No detectable accumulations of silver grains were observed in association with diffuse plaques in AD (asterisk, C), amyloid-containing vessels in CAA (empty arrows, D), tangles in Tg4510 mouse brains (empty arrows, E), Pick bodies in a PiD case (empty arrows, F), astrocytic plaques in a PSP case (empty arrows, G) or coiled bodies and globose tangles in a CBD case (empty arrows, H). No silver grains were detected in association with Lewy bodies in a DLB case (empty arrows, I), α-synuclein inclusions in a MSA case (empty arrows, J) or TDP-43 inclusions in a FTLD-TDP-43 case (empty arrows, K). Abbreviations: Aβ = amyloid-β, AD = Alzheimer’s disease; APP = amyloid precursor protein, CAA = cerebral amyloid angiopathy, CBD = corticobasal degeneration, DLB = dementia with Lewy bodies, FTLD = frontotemporal lobar degeneration, H&E = hematoxylin and eosin, MSA = multiple system atrophy, PHF = paired helical filament, PiD = Pick’s disease, PSP = progressive supranuclear palsy, TDP-43 = TAR DNA binding protein 43. Scale bar = 2 mm (A), 20 μm (B), 50 μm (C–K).