FIG. 1.

TCDD-elicited metabolomic disruption. Metabolic pathway enrichment analysis (MetPA) of altered metabolites (P ≤ .05) in (A) hepatic and (B) serum extracts. Enrichment values ≥ 4.0 were considered significant and impact scores were used to rank pathways. C, Significantly enriched (P ≤ .05) KEGG pathways for the 3406 DEGs (|fold-change| ≥ 1.5, P1(t) ≥ .8). D, Venn diagram of genes with AhR enrichment within its genomic region and 10 Kb upstream of the TSS determined by ChIP-Seq (FDR ≤ 0.05), putative dioxin response elements (pDREs), and DEGs. Genes possessing AhR enrichment, pDREs, and differential expression are shown. The number of genes meeting these criteria where the AhR enrichment peaks overlap a putative DRE is shown in parentheses. E, Heat map of DEGs associated with lipid transport, processing and metabolism. The presence of AhR enrichment at 2-h post-treatment and/or pDREs is indicated in last column on the right.