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. 2015 Nov 24;149(2):372–384. doi: 10.1093/toxsci/kfv249

FIG. 1.

FIG. 1.

DCLF treatment caused an increase in intracellular Ca++. HepG2 cells were exposed to DCLF (250 μM) or its VEH, alone or in combination with TNF (10 ng/ml) and/or IFN (10 ng/ml) for (A) 6 or (B) 12 h. Cells were then removed from the plate with trypsin and incubated with the Ca++ indicator fluo-3 for 30 min. Intracellular Ca++ levels were quantified by measuring fluo-3 fluorescence intensity by flow cytometry. a, significantly different from corresponding bar in the VEH group. b, significantly different from corresponding bar in the TNF group. c, significantly different from corresponding bar in the IFN group. d, significantly different from Control within a cytokine treatment group. Data are represented as mean ± SEM of at least 3 experiments. Abbreviations: VEH, vehicle; TNF, tumor necrosis factor-alpha; IFN, interferon-gamma; DCLF, diclofenac.