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Properties of YC variants in vitro.(A) Fluorescence anisotropy of YC variants (YC3.12, YC3.20, YC3.30, YC3.60, YC3.70, and YC3.900) at zero and saturated Ca2+. (B) Ca2+ titration curves of YC2.60 (triangles), YC3.60 (circles), and YC4.60 (squares) at pH 7.4. (C) pH titration curves of YC3.60 at zero and saturated Ca2+.
