Fig. 5.

Fast Ca²⁺ imaging of a hippocampal brain slice from a YC3.60_pm-producing transgenic mouse. (A) A low-magnification image of brains of a WT mouse and a transgenic line (no. 62) (TG). A 480DF30 excitation filter and a 535AF25 emission filter were used. (Scale bar = 0.5 mm.) (B) A high-magnification fluorescence image in the CA1 region of the transgenic mouse. (Scale bar = 50 μm.) (C) A bright-field image of hippocampal slice. Electrodes for stimulation (stim) and field recording (f.p.) are described by broken lines. (Scale bar = 0.2 mm). (D) A series of pseudocolored images showing a Ca²⁺ transient. The number in each image shows the time after the start of imaging. Two regions of interest were selected within regions CA1 and DG. (E) The field potential (f.p.) change induced by tetanus. (F) The time course of [Ca²⁺]_pm observed in area CA1 of the transgenic line. (G) The time course of [Ca²⁺]_pm observed in area DG of the transgenic line. (H) The time course of [Ca²⁺]_pm observed in area CA1 of a WT mouse. (E–H) Averaged traces from eight challenges.