TABLE 1.

Crystallization, data collection, and refinement

		MazF-d(AUACAUA)	MazFE24A - MazE(68–82)	MazFE24A - MazE(68–82)
Crystallization
Protein solution		18 mg/ml (0.7 mm) EcMazF + 4.2 mm oligonucleotide in 50 mm phosphate, pH 7.0, 150 mm NaCl	10 mg/ml (0.4 mm) EcMazFE24A + 2.5 mm MazE (68–82) in 20 mm Tris-HCl, pH 7.0, 150 mm NaCl	10 mg/ml (0.4 mm) EcMazFE24A + 2.5 mm MazE (68–82) in 20 mm Tris-HCl, pH 7.0, 150 mm NaCl
Reservoir solution		0.2 m CH3COONa, 0.1 m Tris-HCl, pH 8.5, 30% PEG 4000	0.2 m MgCl, 0.1 m (CH3)2AsO2Na, pH 6.5, 50% PEG6000	24% PEG1500, 20% glycerol
Drop contents		0.1 + 0.1 μl	0.1 + 0.1 μl	0.1 + 0.1 μl
Data collection
Space group		P3121	C2	P61
Cell dimensions		114, 114, 47.5	68.8, 32.2, 86.3	52.2, 52.2,198
		90, 90, 120	90, 97.7, 90	90, 90, 120
Resolution range		47.51–2.90 (3.07–2.90)	42.74–1.63 (1.73–1.63)	44.04–2.48 (2.63–2.48)
Rsym		0.224 (0.778)	0.105 (0.732)	0.087 (0.883)
I/σ (I)		7.62 (2.10)	11.10 (2.11)	10.83 (1.64)
Completeness (%)		92.2 (92.4)	95.9 (90.9)	98.7 (98.4)
Unique reflections		7,461 (1130)	22,847 (3469)	10,603 (1,681)
Total no. of reflections		40,771 (10,292)	82,883 (12,312)	39,042 (6,268)
Wavelength (Å)		0.9801	0.9801	0.9801
Refinement
Rwork/Rfree		0.223/0.290	0.178/0.236	0.197/0.248
Root mean square deviation	Bond angles (°)	0.73	1.05	1.23
Root mean square deviation	Bond lengths (Å)	0.004	0.020	0.010
Content asymmetric unit		1.5 MazF dimers + 3 DNA fragments	1 MazF dimer + 1 MazE peptide	1 MazF dimer + 1 MazE peptide
No. of atoms	Protein	2,558	1,500	1,592
	Solvent	96	663	33
	Other	152
Average B-factor (Å2)	All atoms	38.32	25.31	68.25
	Protein	38.47	23.11	68.29
	Solvent	25.95	45.19	61.75
Ramachandran profile (%)	Core region	97.0	98.7	98.1
	Additional allowed	3.0	1.3	1.9
	disallowed
PDB code		5CR2	5CQX	5CQY