FIGURE 2.

In vivo analysis of EF-P-dependent PPX motifs found in swarming genes. Translation efficiency of chromosomally inserted GFP_PPX reporters were tested in the B. subtilis Δefp and efp^K32A strains and compared with the wild type. GFP_PPX expression was assayed in cells grown in LB in the presence of 1 mm IPTG and harvested during exponential phase. Fluorescence was normalized to A₆₀₀, and error bars represent the mean ± S.D. from three biological replicates. A Student's t test was performed between Δefp and efp^K32A strains within the same motif, indicating that PPV was significantly different (*, p = 0.0137).