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. 2004 Jul 12;101(29):10608–10613. doi: 10.1073/pnas.0403412101

Fig. 1.

Fig. 1.

Elimination of Met in the liver of mice. (A) Schematic representation of the targeting strategy used to generate the Metflox allele. Exon 15 of Met (indicated in red) encodes the ATP-binding site of the Met kinase. Yellow boxes indicate exons 14 and 16; loxP sites are shown by arrowheads. NEO, neomycin resistance gene; BHI, BamHI. (B) Southern blot analysis of genomic DNA from the liver of Mx-Cre; Metflox/+ (lanes 1 and 3) or Mx-Cre; Metflox /– (lanes 2 and 4) animals before (lanes 1 and 2) and after (lanes 3 and 4) pIpC injections. (C) Autophosphorylation of Met precipitated from liver extract of Mx-Cre; Metflox /+ (lane 1) or Mx-Cre; Metflox /– (lanes 2 and 3) 2 days after pIpC injections.