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. 2016 Mar 25;291(21):11042–11054. doi: 10.1074/jbc.M115.713156

FIGURE 5.

FIGURE 5.

Analysis of the lipid-phosphate products by normal phase LC and ESI-MS/MS. A–C, the XICs of the NPLC, the ESI-MS/MS spectra, and the fragmentation schemes of the [M-H]− ions of the Dol-P (P1:C-60:σ4) generated by the A. fulgidus AglB-L (A), the Dol-P (P1:C-50:σ5) generated by the P. calidifontis membrane fractions (B), and the Dol-P (P1:C-45:σ5) generated by the S. solfataricus membrane fractions (C). The fragmentation patterns in the MS/MS spectra indicated that the lipid part is dolichol.