FIGURE 3.
H3K36 methylation is essential for the Neurospora circadian clock. A, Western blot analysis shows that the circadian oscillation of FRQ in set-2KO and H3K36R is impaired when compared with wild-type strain. Samples were grown in constant darkness (DD) for indicated hours before harvest. The Coomassie Blue-stained membranes (mem) represent total protein in each sample and act as a loading control. Quantification of the FRQ protein is shown on the right side of Western blot. Northern blot analysis shows that frq (B) and ccg-1 (C) transcription are arrhythmic in set-2KO and H3K36R strains. Ribosome RNA (rRNA) bands stained by ethidium bromide shown below the Northern blot act as a loading control for each sample. Quantification of frq and ccg1 are shown on the right side of Northern blot. These experiments were performed at least three times, and one representative result is shown here.