FIGURE 6.

The mimic acetylation states of histone H3 regulate frq transcription. A, H3K9QK14QK18Q strain lost the robust conidiation rhythm compared with WT in the race tube assay. Three independent H3K9QK14QK18Q strains were shown in the figure. B, analyses of luciferase activity in wild-type, frq-luc and H3K9QK14QK18Q, frq-luc strains. Three independent H3K9QK14QK18Q, frq-luc strains were used to test the luciferase activity and all strains lost the rhythmic luciferase activity compared with wild-type. One wild-type, frq-luc and one H3K9QK14QK18Q, frq-luc strain result was shown in the figure. C, Western blot analysis showing the circadian oscillation of FRQ in the wild-type and H3K9QK14QK18Q mutant strains. Samples were grown in constant darkness (DD) for indicated hours before harvest. The Coomassie Blue-stained membranes (mem) represent total protein in each sample. The experiment was performed independently at least three times, and one representative result was shown in the figure. D, Western blot analyses were performed using antibodies against FRQ or WC-1 in the wild-type, H3K9QK14QK18Q, wc-1^RIP, and H3K9QK14QK18Q wc-1^RIPstrains. Samples were grown on constant light before harvest and three different H3K9QK14QK18Q wc-1^RIP strains were used. The Coomassie Blue-stained membranes (mem) represent total protein in each sample.