FIGURE 3.

LPS-induced CCL2 secretion is mediated by NF-κB. A, confluent monolayers of M1+FXYD5 cells received either 100 nm of the indicated inhibitor or diluent. 30-min later the cultures received 100 ng/ml LPS and were incubated for 24 h. CCL2 secretion was analyzed in the culture medium. The asterisk indicates a significant difference between the two test groups, as analyzed by ANOVA; ****, p < 0.0001. B, M1 and M1+FXYD5 cells were transfected with NF-κB-LUC and Renilla plasmids. After 24 h the cells were treated with 100 ng/ml LPS for 30 min at 37 °C. Luciferase activity was normalized to Renilla expression. Means ± S.E. of at least three independent experiments are depicted.