FIGURE 8.

FXYD5 is up-regulated in LPS-stimulated mice. A and B, LPS-stimulated secretion of CCL2 and TNFα in lung BALF. Mice were treated with a single intraperitoneal injection of 50 μl of PBS or LPS (6 mg/kg) in 50 μl of PBS and BAL was collected at the indicated times as described under “Experimental Procedures.” The asterisk indicates a significant difference between the two test groups, as analyzed by ANOVA; **, p ≤ 0.01. C, lungs were lysed, and the presence of FXYD5 was analyzed by Western blotting (upper panel). Densitometry analysis of the bands was performed, and the FXYD5 signal was normalized to β-actin (lower panel). Data are expressed as means ± S.E. n = 3. The asterisk indicates a significant difference between the two test groups, as analyzed by ANOVA; *, p ≤ 0.05.