Figure 8. Meiotic Cohesin.

DSB data are shown in red, Rec8 data in black, and Mcd1 data in grey.

(A) Ratios for meiotic cohesin are compared to mitotic cohesin in SK1 for kilobasepairs 440–461 on Chromosome XII. For the mitotic culture, cells were arrested with nocodazole. For meiotic cells, timepoints were collected every 2 h from hour 4 to hour 12 after transfer to SPM. The median ratio value was used to represent the data. Meiosis is slower in an SK1 strain with an HA-epitope-tagged Rec8 than in a wild-type strain (see Figure 9). The gene structure for this locus is shown below the graph, with genes encoded by the Watson strand labeled on top and genes encoded by the Crick strand labeled on the bottom.

(B) Ratios for meiotic cohesin are compared to mitotic cohesin and DSBs for kilobasepairs 295–345 on Chromosome XII.