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. 2016 Mar 21;291(21):11124–11132. doi: 10.1074/jbc.M116.716431

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© 2016 by The American Society for Biochemistry and Molecular Biology, Inc.

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FIGURE 3. — Fatty acid oxidation in skeletal muscle. A, semi-quantitative RT-PCR analysis was used to determine mRNA transcripts of genes involved in fatty acid oxidation in the skeletal (Sk.) muscle of 6-month-old male Cc1^+/+ (open bar) and Cc1^−/− (solid bar) mice. Genes examined are as follows: uncoupled protein 3 (Ucp3, panel i), carnitine palmitoyltransferase 1B (Cpt1b, panel ii), cyclooxygenase-2 (Cox2, panel iii), and pyruvate dehydrogenase kinase isozyme 4 (Pdk4, panel iv). mRNA levels were analyzed in triplicate per mouse and normalized to Gapdh content. Data are expressed as means ± S.E. *, p < 0.05 versus Cc1^+/+ (open bars). B, fatty acid oxidation was assayed in skeletal muscle isolated from fasted 6-month-old Cc1^+/+ (open bar) and Cc1^−/− (solid bar) mice (n >7 per genotype). Data are expressed as mean ± S.E. *, p < 0.05 versus Cc1^+/+ (open bars).