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Snapshots at 10-ns intervals of the molecular dynamics simulations of the complexes between phorbol ester and the RasGRP2 C1 domain. A, wild type. B, quadruple mutant. For easier visualization, the C20, C3, and C4 atoms of the phorbol ester are colored green, violet, and blue. The oxygens on these three carbons are the critical contributors to binding.
